Cell Line



BCRC Number: 60037 Add To Bookmark
Name: HT 1080
Type: Human fibrosarcoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 35 years
Gender: Male
Tissue: connective tissue; fibrosarcoma

Morphology: Epithelial
Split Ratio: 1:4 to 1:8
Isoenzyme Analysis: NP, LD, G6PD, MD, MPI, Pep B and AST
Mycoplasma Test: Negative
Karyology: Pseudodiploid
Virus susceptibility: poliovirus 1; vesicular stomatitis (Indiana); RD114; feline leukemia virus (FeLV)
Tumorigenic: yes, in immunosuppressed mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% heat-inactivated fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from fibrosarcoma arising adjacent to the acetabulum of a 35-year-old Caucasian male; the cells contain an activated N-ras oncogene
Others: STR-PCR profile — D7S820:9, 10 CSF1PO:12 TH01:6 D13S317:12, 14 D16S539:9, 12 vWA:14, 19 TPOX:8 Amelogenin:X, Y D5S818:11, 13

Image / Description:   200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: Cancer 33: 1027-1033, 1974
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