Cell Line

BCRC Number: 60036 Add To Bookmark
Name: P3/NSI/1-Ag4-1 (NS-1)
Type: Mouse BALB/c myeloma, non-secreting clone of P3X63Ag8
Species: Mus musculus (mouse)
Strain Ethnicity: BALB/c
Gender: unknown
Tissue: myeloma; plasmacytoma; B lymphocyte

Morphology: Lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium, Do not allow the cell density to exceed 1 X 10(6) cells/ml
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: every 2 to 3 days

Description: This is a non-secreting clone of P3X63Ag8; kappa chains are synthesized but not secreted; the cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium; the cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity
Product Character: immunoglobulin (not secreted)

Image / Description:   200倍視野下低細胞密度

Biosafety Level: 1
Reference: Eur. J. Immunol. 6: 292-295, 1976; Exp. Cell Res. 60: 61-77, 1970; Eur. J. Immunol. 6: 511-519, 1976; Nature 249: 573-574, 1974; J. Mol. Biol. 90: 691-701, 1974
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