Cell Line

BCRC Number: 60034 Add To Bookmark
Name: Sp2/0-Ag14
Type: Mouse hybridoma, non-secreting
Species: Mus musculus (mouse )
Strain Ethnicity: BALB/c (B cells); BALB/c (myeloma)
Gender: unknown
Tissue: Hybridoma; B lymphocyte

Morphology: Lymphoblast
Split Ratio: maintain cell density between 1 x 104 to 1 x 106 viable cells/ml
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1 x 105 viable cells ml
Medium Renewal: every 2 to 3 days

Description: formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma; the cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 ug/ml and are HAT sensitive.
Product Character: Antigen Expression:H-2d

Image / Description:   200倍視野下低細胞密度

Biosafety Level: 1
Reference: Nature 276: 269-270, 1978; J. Immunol. 126: 317-321, 1981
Frozen Vial for all NTD$ 6000 Order this item

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