Cell Line



BCRC Number: 60031
Name: B16-F10
Type: skin melanoma
Species: Mus musculus (mouse)
Strain Ethnicity: C57BL/6J
Gender: unknown
Tissue: melanoma

Morphology: mixture of spindle-shaped and epithelial-like cells
Split Ratio: 1:10
Isoenzyme Analysis: NP, G6PD, MD, MPI, Pep B, AST, and LD
Mycoplasma Test: negative
Tumorigenic: yes, in syngeneic mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days


Image / Description:   200倍視野下低細胞密度
  200倍視野下高細胞密度
  No Description.
  No Description.
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 60: 1217-1222, 1978
Shipping/Field/Price:
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