Cell Line



BCRC Number: 60030
Name: B16-F1
Type: Mouse melanoma
Species: Mus musculus (mouse)
Strain Ethnicity: C57BL/6J
Gender: unknown
Tissue: Melanoma

Split Ratio: 1:10
Isoenzyme Analysis: NP, G6PD, MD, MPI, Pep B, AST ,and LD
Mycoplasma Test: Negative
Tumorigenic: yes, in syngeneic mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days


Image / Description:   200倍視野下高細胞密度
  200倍視野下低細胞密度
 

Biosafety Level: 1
Reference: Cancer Res. 35: 218-224, 1975; Cancer Res. 36: 3608-3615, 1976; Cancer Res. 37: 3945-3956, 1977; Science 197: 893-895, 1977; J. Natl. Cancer Inst. 60: 1217-1222, 1978; Nat. New Biol. 242: 148-149, 1973
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

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