Cell Line



BCRC Number: 60027
Name: HL-60
Type: Human promyelocytic leukemia
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 36 years
Gender: Female
Tissue: leukemia; acute promyelocytic

Morphology: lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Pseudodiploid
Tumorigenic: yes, form colonies in semi-solid media and produce subcutaneous myeloid tumors in nude mice.
Receptor expressed: complement; Fc

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 80% Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate+ 20% FBS (improve growth)
Growth Property: Suspension; NOTE: The cells initially grow slowly after thawing (doubling time of 72 hours). Patience is required.
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Description: this is a promyelocytic cell line derived from a 36-year-old Caucasian female with acute promyelocytic leukemia; the cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.
Others: STR-PCR profile — D7S820:11,12 CSF1PO:13, 14 TH01:7, 8 D13S317:8, 11 D16S539:11 vWA:16 TPOX:8, 11 Amelogenin:X D5S818:12
Product Character: tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid

Image / Description:   200倍視野下低細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: Nature 270: 347-349, 1977; Blood 54: 713-733, 1979; Proc. Natl. Acad. Sci. USA 75: 2458-2462, 1978 J. Biol. Chem. 260: 2345-2354, 1985
Shipping/Field/Price:
growing cell in 15ml tube for all NTD$ 6000 Order this item

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