Cell Line

BCRC Number: 60026 Add To Bookmark
Name: Neuro-2a
Type: Mouse neuroblastoma
Species: Mus musculus (mouse)
Strain Ethnicity: A
Gender: unknown
Tissue: brain; neuroblastoma; neuroblast

Morphology: Neuronal and amoeboid stem cell
Split Ratio: 1:3 to 1:6
Isoenzyme Analysis: NP, G6PD, MD
Mycoplasma Test: Negative
Karyology: Karyotype unstable within a stemline range of 94 to 98
Virus resistance: poliovirus 1
Virus susceptibility: vesicular stomatitis (Indiana); herpes simplex

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% MEM (Eagle) with Earle's BSS, 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% FBS
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Isolated from a spontaneous tumor of a strain A albino mouse; the cells produces large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cell; the line has been been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, and the synthesis and assembly of microtubular protein .
Product Character: tubulin; acetylcholinesterase

Image / Description:   200倍視野下低細胞密度
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Biosafety Level: 1
Reference: J. Cell. Biol. 43: 69A, 1969; Proc. Natl. Acad. Sci. USA 65: 129-136, 1970
Frozen Vial for all NTD$ 6000 Order this item

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