Cell Line



BCRC Number: 60023
Name: MRC-5
Type: embryonal lung, normal, human
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 14 weeks gestation
Gender: Male
Tissue: Lung; normal; fibroblast

Morphology: Fibroblast
Split Ratio: 1:2 to 1:5
Isoenzyme Analysis: NP, G6PD, MD, MPI, Pep B, AST and LD
Mycoplasma Test: Negative
Cytogenetics: diploid; 46,XY
Karyology: Normal human male; diploid; stable
Virus susceptibility: poliovirus 1; herpes simplex; vesicular stomatitis (Indiana)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from normal lung tissue of a 14-week-old male fetus; the cells are capable of 42 to 46 population doublings before the onset of senescence.
Others: STR-PCR profile — D7S820:10, 11 CSF1PO:11, 12 TH01:8 D13S317:11, 14 D16S539:9, 11 vWA:15 TPOX:8 Amelogenin:X, Y D5S818:11, 12

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Biosafety Level: 1
Reference: Nature 227:168-170, 1970; Nature 238:26-30, 1972; Nature 239:316-318, 1972
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