Cell Line



BCRC Number: 60019
Name: 293
Type: Human embryonic kidney, adenovirus type 5 transformed
Species: Homo sapiens (human)
Age Stage: Embryo
Gender: unknown
Tissue: Kidney; transformed with adenovirus 5 DNA

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP, G6PD, MD
Mycoplasma Test: Negative
Cytogenetics: hypotriploid
Karyology: Hypotriploid; modal number = 64
Virus susceptibility: very sensitive to human adenoviruses
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Receptor expressed: vitronectin

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% Fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:11, 12 CSF1PO:12 TH01:7, 9.3 D13S317:12, 14 D16S539:9, 13 vWA:16, 19 TPOX:11 Amelogenin:X D5S818:8
Product Character: as name as HEK-293

Image / Description:   200倍視野下低細胞密度
  200倍視野下高細胞密度
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Use Restriction: These cells are distributed for research purposes only. Please direct any commercial license inquiries to AdVec Inc.
Reference: J. Gen. Virol. 36:59-72, 1977; Virology 77:319-329, 1977; ibid., 86:10-21, 1978; J. Biol. Chem. 265: 5938-5941, 1990; Virology 233: 423-429, 1997
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