Cell Line

BCRC Number: 60014 Add To Bookmark
Name: IMR-32
Type: Human neuroblastoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 13 months
Gender: Male
Tissue: brain; neuroblastoma; neuroblast

Morphology: Fibroblast and neuroblast
Split Ratio: 1:3 to 1:6
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Cytogenetics: Stable male karyotype with stemline number of 49.
Karyology: Modal number = 49
Virus resistance: echovirus 11
Virus susceptibility: vesicular stomatitis (Indiana); herpes simplex; vaccinia; coxsackievirus B3; poliovirus 3 (poorly)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) with Earle's BSS, 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA, Don't allow the cells to be too confluent, otherwise the cell will be detach from the flask!!
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Established from an abdominal mass occurring in a 13-month-old Caucasian male; the tumor was diagnosed as a neuroblastoma with rare areas of organoid differentiation; the culture is a mixture of two morphologically distinct cell types, the predominant one is a small neuroblast-like cell.The other is a large hyaline fibroblast.
Others: STR-PCR profile — D7S820:9, 10 CSF1PO:11, 12 TH01:7, 9.3 D13S317:9 D16S539:8 vWA:15 TPOX:11 Amelogenin:X, Y D5S818:11, 12

Image / Description:   200倍視野下低細胞密度
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Biosafety Level: 1
Reference: Cancer Res. 30: 2110-2118, 1970
Frozen Vial for all NTD$ 6000 Order this item

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