Cell Line



BCRC Number: 60011
Name: Sf9
Type: Fall armyworm ovary
Species: Spodoptera frugiperda (fall armyworm)
Age Stage: Pupa
Gender: Female
Tissue: ovary, pupa

Morphology: round cell
Split Ratio: 1:5 or greater
Isoenzyme Analysis: NP, LDH, G6PD, MD, MPI, PESB, AST
Mycoplasma Test: Negative
Virus susceptibility: Autographa californica (MNPV); baculoviruses; St. Louis encephalitis (SLE)

Incubation Temp(°C): 28°C
Incubation Condition: without CO2 in air
Culture Medium: 90% Grace's Insect Medium with L-glutamine that contains 500 mg/L calcium chloride, 2800 mg/L potassium chloride, 3330 mg/L lactalbumin hydrolysate, 3330 mg/L yeastolate + 10% heat-inactivated fetal bovine serum
Growth Property: Suspension and weakly attachment
SubCulture Procedure: by pipetting across the monolayer or by hitting the flask against the palm of your hand; if many floating cells are present before subculturing, the old medium and the floating cells may be discarded and the medium replaced before subculture
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Cloned from the parent line, IPLB-SF21 AE, which was derived from pupa ovarian tissue of the fall armyworm, Spodoptera frugiperda; the line can be used to replicate baculovirus expression vectors.

Image / Description:   high density
  high density
 

Biosafety Level: 1
Reference: In Vitro 13: 213-217, 1977; Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985
Shipping/Field/Price:
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