Cell Line

BCRC Number: 60007
Name: K-562
Type: Human chronic myelogenous leukemia
Species: Homo sapiens (human)
Age Stage: 53 years
Gender: Female
Tissue: bone marrow; chronic myelogenous leukemia (CML)

Morphology: Lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP; LD; G6PD; MD; MPI; Pep B; AST
Mycoplasma Test: Negative
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Description: Established from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises; the cell population has been characterized as highly undifferentiated and of the granulocytic series; the line has attained widesprea use as a highly sensitive in vitro target for the natural killer assay.
Others: STR-PCR profile — D7S820:9, 11 CSF1PO:9, 10 TH01:9.3 D13S317:8 D16S539:11, 12 vWA:16 TPOX:8, 9 Amelogenin:X D5S818:11, 12

Image / Description:   100倍視野下

Biosafety Level: 1
Reference: Blood 45: 321-334, 1975; Blood 56: 344-350, 1980; Leukemia Res. 3: 363-370, 1979; Int. J. Cancer 23: 143-147, 1979; Proc. Soc. Exp. Biol. Med. 166: 546-550, 1981; J. Natl. Cancer Inst. 59: 77-83, 1977; Exp. Hematol. 11: 601-610, 1983
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