Cell Line



BCRC Number: 60006
Name: CHO-K1
Type: Chinese hamster ovary
Species: Cricetulus griseus (hamster, Chinese)
Age Stage: Adult
Gender: Female
Tissue: Ovary

Morphology: Epithelial
Split Ratio: 1:4 to 1:8
Isoenzyme Analysis: NP; LD; G6PD; MD; MPI; Pep B; AST
Mycoplasma Test: Negative
Cytogenetics: hypodiploid
Karyology: Chromosome Frequency Distribution 50 Cells: 2n = 22
Virus resistance: poliovirus 2; modoc virus; Button Willow virus
Virus susceptibility: vesicular stomatitis (Indiana); Getah virus

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Ham's F-12K medium + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: Once or twice between subculture

Description: derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster; the cells require proline in the medium for growth

Image / Description:   100倍視野下高細胞密度
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Patent Statement: U.S. and/or other patent application
Reference: J. Exp. Med. 108: 945-955, 1958; Proc. Natl. Acad. Sci. USA 52: 288, 1965; ibid. 60:1275-1281, 1968; ibid. 64: 1284, 1969; Genetics 55: 513-524, 1967; Science 164: 312-314 1969
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