Cell Line



BCRC Number: 60004
Name: MDCK
Type: Canine kidney, normal
Species: Canis familiaris (dog) Synonym(s): canine
Strain Ethnicity: Cocker spaniel
Age Stage: Adult
Gender: Female
Tissue: Kidney; normal

Morphology: Epithelial
Split Ratio: 1:2 to 1:6
Isoenzyme Analysis: NP; LD; G6PD; MD; MPI; Pep B; AST
Mycoplasma Test: Negative
Virus resistance: poliovirus 2; coxakievirus B3, B4
Virus susceptibility: vaccinia; coxsackievirus B5; reovirus 2,3; adenovirus 4,5; vesicular exanthema of swine; infectious canine hepatitis

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) with Earle's BSS, 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from a kidney of an apparently normal adult female Cocker Spaniel; the cells are positive for keratin by immunoperoxidase staining
Product Character: keratin

Image / Description:   No Description.
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Biosafety Level: 1
Reference: Proc. Soc. Exp. Biol. Med. 122: 931-935, 1966
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