Cell Line



BCRC Number: 60003 Add To Bookmark
Name: STO
Type: Mouse embryonic ; fibroblast
Species: Mus musculus (mouse)
Age Stage: Embryo
Gender: unknown
Tissue: Embryo; fibroblast

Morphology: Fibroblast
Split Ratio: 1:3 to 1:10
Isoenzyme Analysis: NP; LD; G6PD; MD; Pep B; MPI; AST
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from SIM mouse embryonic fibroblast line; the cells are resistant to 6-thioguanine and ouabain and are HGPRT- (HPRT-) and HAT sensitive; the line is used to prepare feeder layers by irradiation or mitomycin C treatment.

Image / Description:   200倍視野下低細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975; Cell 6: 467-474, 1975; Dev. Biol. 61: 230-244, 1977
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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