Cell Line



BCRC Number: 60001 Add To Bookmark
Name: RAW 264.7
Type: Mouse BALB/c macrophage, Abelson murine leukemia virus transformed
Species: Mus musculus (mouse)
Strain Ethnicity: BALB/c
Gender: Male
Tissue: macrophage; monocyte; Abelson murine leukemia virus-induced tumor

Morphology: Monocyte/macrophage
Split Ratio: 1:3 to 1:6
Isoenzyme Analysis: NP; G6PD; MD; MPI; Pep B; AST; LD
Mycoplasma Test: Negative
Receptor expressed: complement (C3)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: by scraping
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Established from a tumor induced by Abelson murine leukemia virus; they are negative for surface immunoglobulin, Ia and Thy-1.2; the cells do not secrete detectable virus particles and negative in the XC plaque formation assay; the cells will pinocytose latex beads and zymosan.
Product Character: lysozyme

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Biosafety Level: 1
Reference: J. Immunol. 119: 950-954, 1977; Cell 15: 261-267, 1978
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