Clone



 
BCRC Number: 43079
Organism: pCR2.1-TOPO vector+82-2-1
Chinese Name: 沈原民
History: << BCRC << insert of plant virus sequence in Taichung District Agricultural Research and Extension Station+invitrogen pCR2.1-TOPO vector
Description: Tomato yellow leaf curl Thailand virus, Beidou isolate
DNA: Tomato yellow leaf curl Thailand virus, Beidou isolate
Construct(kb): 6.69
Insert Size(kb): 2.76
Vector Name: invitrogen pCR2.1-TOPO vector
Selection Marker: ampicillin or kanamycin
Suggest Host: Escherichia coli
Shipped Host: Escherichia coli
Insert Gene Name: Tomato yellow leaf curl Thailand virus, Beidou isolate
Insert Gene Source: Tomato yellow leaf curl Thailand virus, Beidou isolate
Image / Description:   存活測試結果
 
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Special Requirement: 限研究試驗用:申請分讓者瞭解並同意本生物物質限試驗或研究用。受分讓者欲對本生物物質、複製物或修飾物進行營利使用時,應與本生物物質提供者另行簽訂契約並協商利益回饋。For experimental or research purposes only: If the recipient wishes to use the biological material, duplicates, or derivatives for commercial purposes, the recipient shall negotiate with the depositor for another agreement.
Author: Shen, Yuan-min
Other Collection No.: Taichung District Agricultural Research and Extension Station #82-2-1
Shipping/Field/Price:
Freeze-Dried for all NTD$ 2500 Order this item


References



P458
W. S. Tsai, S. L. Shih, L. Kenyona, S. K. Green and F.-J. Jan 2011. Temporal distribution and pathogenicity of the predominant tomato-infecting begomoviruses in Taiwan Plant Pathology 60: 787-799.
Keyword
begomovirus evolution, Geminiviridae, phylogenetic analysis, Solanum lycopersicum
 

Medium



275
YEAST-TRYPTONE MEDIUM (LB Medium) with 50μg/mL Kanamycin
Distilled water1.0 L
Kanamycin50.0 mg
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

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