Clone



 
BCRC Number: 43075
Organism: Escherichia coli pMG-CAhydA
Chinese Name: 羅壽鎮
History: BCRC << C.C. Huang << ATC C<< C.S. Harwood << R.K. Clayton
Description: promoter PpckA, 576 bps, cloned from promoter region of phosphoenolpyruvate carboxykinase gene of Rhodopseudomonas palustris 636 (BCRC 16636); CAhydA, 1835 bps, cloned form FeFe-hydrogenase gene of Clostridium acetobutylicum ATCC 824; terminator TpckA, 52 bps, synthesized base on terminator region of phosphoenolpyruvate carboxykinase gene of Rhodopseudomonas palustris NO.7.
Source: BCRC << C.C. Huang << ATCC << C.S. Harwood << R.K. Clayton
Construct(kb): 8
Vector Name: pMG-CAhydA
Selection Marker: Kanamycin
Suggest Host: Rhodopseudomonas palustris/E.coli
Shipped Host: E.coli
Image / Description:   No Description.
 
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Special Requirement: 須獲提供者同意:本生物物質須經提供者審酌個別分讓申請,經取得提供者同意後始得分讓。Depositor's approval required: The biological material will be available upon approval from the depositor on a case-by-case basis.
Author: Lo, Shou-Chen
Shipping/Field/Price:
Freeze-Dried for all NTD$ 2500 Order this item


References



 

Medium



873
YEAST-TRYPTONE MEDIUM (LB Medium) with 30 μg/mL Kanamycin
Distilled water1.0 L
Kanamycin30.0 mg
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

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