Clone



 
BCRC Number: 43073
Organism: pBCJ164.3
History: BGSC
Description: Integration vector; facilitates a non-mutational integration at the rpsD locus by a Campbell-type crossover event. Allows the constitutive expression of inserts cloned into the NdeI site.
Construct(kb): 5.1
Insert Size(kb): 5.1
Vector Name: pBCJ164.3
Selection Marker: Amp, Cm
Suggest Host: E. coli, Bacillus subtilis
Shipped Host: E. coli
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Other Collection No.: ECE176
Shipping/Field/Price:
Freeze-Dried for all NTD$ 2500 Order this item


References



P448
Jester, B. C., J. D. Levengood, H. Roy, M. Ibba, and K. M. Devine 2003. Nonorthologous replacement of lysyl-tRNA synthetase prevents addition of lysine analogues to the genetic code Proc. Natl. Acad. Sci. USA 100: 14351-14356.
 

Medium



793
LB medium with 100 μg/mL Ampicillin and 5 μg/mL Chloramphenicol
Ampicillin100 μg/mL
Chloramphenicol 5 μg/mL
Distilled water1.0 L
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

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