BCRC Number: 43072
Organism: pNDH09
History: BGSC
Description: Integration vector that directs insertion of a cassette into the Bacillus subtilis lacA gene. The cassette contains the sortase A gene (srtA) of L. monocytogenes fused to an IPTG-inducible P-spac promoter. Use this plasmid to construct expression hosts for the cell surface anchoring vector, pNDH10.
Insert Size(kb): 7
Vector Name: pNDH09
Selection Marker: Amp
Suggest Host: E. coli and Bacillus subtilis
Shipped Host: E. coli
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Other Collection No.: ECE196
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Nguyen, H. D. and W. Schumann 2006. Establishment of an experimental system allowing immobilization of proteins on the surface of Bacillus subtilis cells. J. Biotechnol. 122: 473-482.


YEAST TRYPTONE MEDIUM (LB Medium) with 100μg/mL Ampicillin
Ampicillin100.0 mg
Distilled water1.0 L
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

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