Clone



 
BCRC Number: 43072
Organism: pNDH09
History: BGSC
Description: Integration vector that directs insertion of a cassette into the Bacillus subtilis lacA gene. The cassette contains the sortase A gene (srtA) of L. monocytogenes fused to an IPTG-inducible P-spac promoter. Use this plasmid to construct expression hosts for the cell surface anchoring vector, pNDH10.
Insert Size(kb): 7
Vector Name: pNDH09
Selection Marker: Amp
Suggest Host: E. coli and Bacillus subtilis
Shipped Host: E. coli
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Other Collection No.: ECE196
Shipping/Field/Price:
Freeze-Dried for all NTD$ 2500 Order this item


References



P447
Nguyen, H. D. and W. Schumann 2006. Establishment of an experimental system allowing immobilization of proteins on the surface of Bacillus subtilis cells. J. Biotechnol. 122: 473-482.
 

Medium



290
YEAST TRYPTONE MEDIUM (LB Medium) with 100μg/mL Ampicillin
Ampicillin100.0 mg
Distilled water1.0 L
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintainance: +886-3-5223191 ext 593