Clone



 
BCRC Number: 43071
Organism: pNDH10
History: BGSC
Description: pNDH10 is a shuttle vector, replicating at high copy number in E. coli in lower copy number in Bacillus subtilis. It features a xylose-inducible expression cassette upstream from the coding sequence of an anchoring protein that allows fused proteins to be anchored to the cell surface of a suitable host.
Insert Size(kb): 8.5
Vector Name: pNDH10
Selection Marker: Amp
Suggest Host: E. coli and Bacillus subtilis
Shipped Host: E. coli
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Other Collection No.: ECE197
Shipping/Field/Price:
Freeze-Dried for all NTD$ 2500 Order this item


References



P447
Nguyen, H. D. and W. Schumann 2006. Establishment of an experimental system allowing immobilization of proteins on the surface of Bacillus subtilis cells. J. Biotechnol. 122: 473-482.
 

Medium



290
YEAST TRYPTONE MEDIUM (LB Medium) with 100μg/mL Ampicillin
Ampicillin100.0 mg
Distilled water1.0 L
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

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