BCRC Number: 43071
Organism: pNDH10
History: BGSC
Description: pNDH10 is a shuttle vector, replicating at high copy number in E. coli in lower copy number in Bacillus subtilis. It features a xylose-inducible expression cassette upstream from the coding sequence of an anchoring protein that allows fused proteins to be anchored to the cell surface of a suitable host.
Insert Size(kb): 8.5
Vector Name: pNDH10
Selection Marker: Amp
Suggest Host: E. coli and Bacillus subtilis
Shipped Host: E. coli
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Other Collection No.: ECE197
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Nguyen, H. D. and W. Schumann 2006. Establishment of an experimental system allowing immobilization of proteins on the surface of Bacillus subtilis cells. J. Biotechnol. 122: 473-482.


YEAST TRYPTONE MEDIUM (LB Medium) with 100μg/mL Ampicillin
Ampicillin100.0 mg
Distilled water1.0 L
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

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