Vector



BCRC Number: 41529 Add To Bookmark
Organism: YIp5(pRB12)
History: << ATCC<< D. Bostein<< R. W. Davis
Description: This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 from lambda590-Sc2904 into the AvaI site of pBR322. The 5' - 3' orientation of URA3 is clockwise; SmaI and AvaI sites in the 3' of URA3 are close to the PvuII site of pBR322.
Other Collection No.: ATCC37061
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Vector Category: YI-type (integrating) shuttle vector ,integrating vector ,shuttle vectorYI-type integrating shuttle vector.
Host Range: Escherichia coli, C600
Replication Origin: pMB1
Select Marker: amp, tet, URA3
Shipped Host: Freeze-dried Escherichia coli HB101
Size(kb): 5.5
Multi Clone Site: No
Shipping/Field/Price:
Freeze-Dried for all NTD$ 3500 Order this item


References



P280
Pouwels, P. H., B. E. Enger-Valk, and W. J. Brammar. 1986. In Cloning Vectors. Vectors for yeasts. Elsevier, New York.
P7
Botstein, D., S. C. Falco, S. E. Stewart, M. Brennan, S. Scherer, D. T. Stinchcomb, K. Struhl, and R. W. Davis. 1979. Sterile host yeasts (SHY) : a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24.

Medium



293
YEAST-TRYPTONE MEDIUM (LB Medium) with 100μg/mL Ampicillin and 15μg/mL Tetracycline
Ampicillin100.0 mg
Distilled water1.0 L
NaCl10.0 g
Tetracycline15.0 mg
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

Tags



Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 300193, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintenance: +886-3-5223191 ext 572

Privacy Policy        Cookie