BCRC Number: 41300 Add To Bookmark
Organism: YIp33(pRB19)
History: << ASIB<< M. Casadabam
Description: A general purpose integrating S. cerevisiae/E. coli shuttle vector. This was constructed by inserting a 2.2 kb SalI/XbaI fragment containing LEU2 from pYe(Leu2) 10 into the SalI site of pBR322. The 5'-3' orientation of LEU2 is counterclockwisre. LEU2, originating from strain X2180-1A, includes a region of Ty1-17.
Other Collection No.: ATCC37064
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Vector Category: shuttle vector, cloning vector, integrating vector, YI-type(integrating) shuttle vector
Host Range: Saccharomyces cerevisiae Escherichia coli
Vector Type: plasmid
Replication Origin: pMB1
Select Marker: amp, LEU2
Suggested Host: rec- strain of E. coli
Shipped Host: Freeze-dried Escherichia coli MC1065
Size(kb): 6.7
Multi Clone Site: Yes
Image / Description:   99年度質體複核結果
Freeze-Dried for all NTD$ 2500 Order this item


Botstein, D., S. C. Falco, S. E. Stewart, M. Brennan, S. Scherer, D. T. Stinchcomb, K. Struhl, and R. W. Davis. 1979. Sterile host yeasts (SHY) : a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24.


YEAST TRYPTONE MEDIUM (LB Medium) with 100μg/mL Ampicillin
Ampicillin100.0 mg
Distilled water1.0 L
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.


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