Vector



BCRC Number: 41300 Add To Bookmark
Organism: YIp33(pRB19)
History: << ASIB<< M. Casadabam
Description: A general purpose integrating S. cerevisiae/E. coli shuttle vector. This was constructed by inserting a 2.2 kb SalI/XbaI fragment containing LEU2 from pYe(Leu2) 10 into the SalI site of pBR322. The 5'-3' orientation of LEU2 is counterclockwisre. LEU2, originating from strain X2180-1A, includes a region of Ty1-17.
Other Collection No.: ATCC37064
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Vector Category: shuttle vector, cloning vector, integrating vector, YI-type(integrating) shuttle vector
Host Range: Saccharomyces cerevisiae Escherichia coli
Vector Type: plasmid
Replication Origin: pMB1
Select Marker: amp, LEU2
Suggested Host: rec- strain of E. coli
Shipped Host: Freeze-dried Escherichia coli MC1065
Size(kb): 6.7
Multi Clone Site: Yes
Image / Description:   99年度質體複核結果
 
Shipping/Field/Price:
Freeze-Dried for all NTD$ 2500 Order this item


References



P7
Botstein, D., S. C. Falco, S. E. Stewart, M. Brennan, S. Scherer, D. T. Stinchcomb, K. Struhl, and R. W. Davis. 1979. Sterile host yeasts (SHY) : a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24.

Medium



290
YEAST TRYPTONE MEDIUM (LB Medium) with 100μg/mL Ampicillin
Ampicillin100.0 mg
Distilled water1.0 L
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

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