BCRC Number: 41182 Add To Bookmark
Organism: pGH101
History: << ATCC
Description: The lacZ gene is without any regulatory elements and lacking 8 non-esssential N-terminal amino acids. The gene product, beta-galactosidase, can be excised with either BamHI and SalI for the study of eukaryotic expression.
DNA: genomic DNA
Construct(kb): 8
Insert Size(kb): 3
Vector Name: pBR322
Selection Marker: amp
Suggest Host: rec- strain of E. coli
Shipped Host: Freeze-dried Escherichia coli HB101
Insert Gene Name: beta-galactosidase
Insert Gene Source: Escherichia coli
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Other Collection No.: ATCC37480
Freeze-Dried for all NTD$ 2500 Order this item


Herman, G. E., W. E. O, Brien, and A. L. Beaudet. 1986. An E. coli @b-galactosidase cassette suitable for study of eucaryotic expression. Nucleic Acids Res. 14: 7130.


YEAST TRYPTONE MEDIUM (LB Medium) with 100μg/mL Ampicillin
Ampicillin100.0 mg
Distilled water1.0 L
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.


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