Fungi



BCRC Number: 34769
Organism: Aspergillus niger
Author: Tieghem
History: << NRRL << S. M. Martin
Other Collection No.: ATCC 11414; IMI 75353; NRRL 2270; VTT D-77050; QM 6891; WB 2270
Source: Derived from NRRL 328 (BCRC 34770) which was isolated from dilution platings of Wis. 72
Local Strain: NO
Characterization:
Degradation apple pomace--M2846
Production polyketide synthase--M2850
polygalacturonase endopolygalacturonase, pectinase--M2847
citric acid--M2836, M2840, M2842, M2843, M2844, M2845, M2846
carnitine acetyltransferase--M2835
aconitic hydrase--M2848
ATP citrate lyase--M2835
6-phosphofructo-2-kinase--M2840
Taxonomy --M2841
Others: genes associated with morphology--M2849; lipid biosynthesis--M2837,M2839; transformation host--M986,M2838
Growth Conditions: 25°C 7 day(s)
Oxygen Requirement: Aerobic
Biosafety Level: 1
Type Strain: NO
Shipping/Field/Price:
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References



M2835
Jernejc, K., A. Perdih, and A. Cimerman. 1991. ATP: citrate lyase and carnitine acetyltransferase activity in a citric-acid-producing Aspergillus niger strain. Appl. Microbiol. Biotechnol. 36: 92-95.
M2836
Jernejc, K., A. Cimerman, and A. Perdih. 1982. Citric acid production in chemically defined media by Aspergillus niger. Eur. J. Appl. Microbiol. 14: 29-33.
M2840
Harmsen, H. J. M., E. M. Kubicek-Pranz, M. Röhr, J. Visser, and C. P. Kubicek. 1992. Regulation of 6-phosphofructo-2-kinase from the citric-acid-accumulating fungus Aspergillus niger. Appl. Microbiol. Biotechnol. 37: 784-788.
M2841
Varga J, F. Kevei, A. Vriesema, F. Debets, Z. Kozakiewicz, and J. H. Croft. 1993. Restriction fragment length polymorphisms in the mitochondrial DNAs of the Aspergillus niger aggregate. Mycol. Res. 97: 1207-1212.
M2842
McIntyre, M. and B. McNeil. 1997. Effect of carbon dioxide on morphology and product synthesis in chemostat cultures of Aspergillus niger A60. Enzyme Microb. Technol. 21: 479-483.
M2843
Perlman, D., D. A. Kita, and W. H. Peterson. 1946. Production of citric acid from cane molasses. Arch. Biochem. 11: 123-129.
M2844
Kubicek, C. P. and M. Rohr. 1977. Influence of manganese on enzyme synthesis and citric acid accumulation in Aspergillus niger. Eur. J. Appl. Microbiol. 4: 167-175.
M2845
Rohr, M., P. J. Stadler, W. O. J. Salzbrunn, and C. P. Kubicek. 1979. An improved method for characterization of citrate production by conidia of Aspergillus niger. Biotechnol. Lett. 1: 281-286.
M2846
Hang, Y. D. and E. E.Woodams. 1984. Apple pomace: a potential substrate for citric acid production by Aspergillus niger. Biotechnol. Lett. 6: 763-764.
M2847
Bailey, M. J. and E. Pessa. 1990. Strain and process for production of polygalacturonase. Enzyme Microb. Technol. 12: 266-271.
M2848
Neilson, N. E. 1962. Aconitic hydrase from Aspergillus niger. Methods Enzymol. 5: 614-616.
M2850
Chiang, Y. M., K. M. Meyer, M. Praseuth, S. E. Baker, K. S. Bruno, C. C. Wang. 2011. Characterization of a polyketide synthase in Aspergillus niger whose product is a precursor for both dihydroxynaphthalene (DHN) melanin and naphtho-γ-pyrone. Fungal Genet. Biol. (in press)
 

Medium



25
MALT EXTRACT AGAR I (Blakeslee's Formula)
Glucose20.0g
Peptone1.0g
Agar20.0g
Distilled water1.0L
Malt extract20.0g
Add glucose prior to sterilization.
36
POTATO DEXTROSE AGAR (PDA)
Diced potatoes200.0 g
Glucose20.0 g
Agar15.0 g
Distilled water1.0 L
Boil finely diced potatoes in 500 ml of water until thoroughly cooked, filter through cheesecloth and add water to filtrate to 1.0 L. The agar is dissolved in the filtrate by heating, and the glucose is added prior to sterilization.

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