Fungi



BCRC Number: 33307 Add To Bookmark
Organism: Rhizopus delemar
Author: (Boidin) Wehmer & Hanzawa emend. A. Abe et al.
History: << ATCC (Rhizopus oryzae) << M. Iwai
Other Collection No.: ATCC 34612
Source: root of Brassica pekinensis
Isolated Info:
    Isolated location: Japan
Characterization:
Production : lipase--M2236,M2250,M2239;
lactic acid (lactate)--M2238,M2241;
glucoamylase --M2241;
fumaric acid --M2241,M2238;
ethyl alcohol (ethanol)--M2241;
C-lipase --M800;
lactic and fumaric acid, ethanol, alpha-amylase, and glucoamylase from cassava --M2241;
Others: genomic library--M2240; cDNA encoding lipase --M2235; stimulation of lipase production by glycerol --M2237;
Growth Conditions: 24°C
Oxygen Requirement: Aerobic
Biosafety Level: 1
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References



M2235
Haas, M. J. et al. 1991. Cloning, expression, and characterization of a cDNA encoding a lipase from Rhizopus delemar. Gene 109: 107-113.
M2236
Iwai, M., and Y. Tsujisaka. 1974. Interconversion of two lipases from Rhizopus delemar. Agric. Biol. Chem. 38: 1249-1254.
M2237
Haas, M. J., and D. G. Bailey. 1993. Glycerol as a carbon source for lipase production by the fungus Rhizopus delemar. Food Biotechnol. 7: 49-73
M2238
Soccol, C. R., V. I. Stonoga, and M. Raimbault. 1994. Production of L-lactic acid by Rhizopus species. World J. Microbiol. Biotechnol. 10: 433-435.
M2239
Chen, J.-P., and S. D. McGill. 1992. Enzymatic hydrolysis of triglycerides by Rhizopus delemar. Food Biotechnol. 6: 1-18.
M2240
Haas, M. J., J. R. Genuario, and S. H. Feairheller. 1990. Construction of a Rhizopus delemar genomic library and screening for direct liase gene expression. Food Biotechnol. 4: 647-661.
M2241
Soccol, C. R., B. Marin, M. Raimbault, and J.-M. Lebeault. 1994. Breeding and growth of Rhizopus in raw cassava by solid state fermentation. Appl. Microbiol. Biotechnol. 41: 330-336.
M2250
Pabai, F., S. Kermasha, and A. Morin. 1995. Interesterification of butter fat by partially purified extracellular lipases from Pseudomonas putida, Aspergillus niger and Rhizopus oryzae. World J. Microbiol. Biotechnol. 11: 669-677
M800
Iwai, M., S. Okumura, and Y. Jsujisaka. 1980. Synthesis of terpene alcohol esters by lipase. Agric. Biol. Chem. 44: 2731-2732.

Medium



25
MALT EXTRACT AGAR I (Blakeslees Formula)
Glucose20.0g
Peptone1.0g
Agar20.0g
Distilled water1.0L
Malt extract20.0g
Add glucose prior to sterilization.

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