Fungi



BCRC Number: 32521 Add To Bookmark
Organism: Rhizopus oryzae
Author: Went & Prinsen Geerligs
History: << CBS << ATCC
Other Collection No.: ATCC 6227b ;CBS 382.52 ;IFO 5781 ;IFO 6154 ;IMI 57762 ;NRRL 12939 ;VKM F-401;IFO 6300;NRRL 12939
Characterization:
Biotransformation : sesquiterpene lactone costunolide--M18;
Production : 17β-hydroxysteroid dehydrogenase--M2067;
11?hydroxylase--M2105, M2106;
chiral alcohols--M2037;
U. S. Pat. 3,053,858
steroids--M2087;
Others: hydroxylation of 17-alpha-ethynyl steroids--M2065;reduce 3-methoxy-8(14)-seco-1,3,5(10),9(11)-estratetraene-14,17-dione--U. S. Pat. 3,562,112;detoxification of steroids--M2401
Mating Type: minus strain
Growth Conditions: 30°C
Oxygen Requirement: Aerobic
Biosafety Level: 1
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References



M18
Clark, A. M., and C. D. Hufford. 1979.. Microbial transformations of the sesquiterpene lactone costunolide. J. Chem. Soc. Perkin Trans. 1979:: 3022-3028..
M2037
Kasai, M., H. Ziffer, and J. V. Silverton 1985. Inantioselective ester hydrolyses using Rhizopus nigricans: stereoselective synthesis and absolute stereochemistry of (-)-cis- and (-)-trans-1-hydroxy-4-methyl-1,2,3,4-tetrahydronaphthalene Can. J. Chem. 63: 1287-1291
M2067
Lanisnik, T., M. Zakelj-Mavric, and I. Belic 1992. Fungal 17@b-hydroxysteroid dehydrogenase FEMS Microbiol. Lett. 99: 49-52
M2087
Abul-Hajj, Y. J 1972. Stereochemistry of C-1,2 dehydrogenation of 5@b-pregnane-3,11,20-trione by Septomyxa affinis J. Biol. Chem. 247: 686-691
M2105
Zakilj-Mavric, M., and I. Belic 1987. Hydroxylation of steroids with 11@-hydroxylase of Rhizopus nigricans J. Steroid Biochem. 28: 197-201
M2106
Hudnik-Plevnik, T., and B. Cresnar 1990. Factors affecting the induction of 11@a-hydroxylase of progesterone in the filamentous fungus Rhizopus nigricans J. Steroid Biochem. 35: 749-751

Medium



186
FLAVOBACTERIUM MEDIUM
Na-caseinate2.0g
Yeast extract0.5g
Proteose peptone1.0g
K2HPO40.5g
Agar15.0g
Distilled water1.0L
Adjust pH to 7.4.

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