Fungi



BCRC Number: 31619
Organism: Penicillium rubens
Author: Biourge
History: << DSM << Friedrich << Demain
Other Collection No.: ATCC 28089 ;DSM 1075
Source: mutant of NRRL 1951
Characterization:
Production of : penicillin V--M2096;
isopenicillin N synthetase--M2022;
acyl-CoA-6-aminopenicillanic acid acyltransferase--M974, M2079, M2080, M2081;
penicillin--M268,M269;
Others: phenylacetic acid transport system--M812,M2383,M2384; re-identified as Penicillium rubens, a closely related species of Penicillium chrysogenum, based on DNA sequence analysis
Growth Conditions: 24°C
Oxygen Requirement: Aerobic
Biosafety Level: 1
Shipping/Field/Price:
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References



M2022
Luengo, J. M., M. T. Alemany, F. Salto, F. Ramos, M. J. Lopez-Nieto, and J. F. Martin 1986. Direct enzymatic synthesis of penicillin G using cyclases of Penicillium chrysogenum and Acremonium chrysogenum BioTechnology 4: 44-47
M2079
Alonso, M. J., F. Bermejo, A. Reglero, J. M. Fernandez-Canon, G. G. de Buitrago, and J. M. Luengo 1988. Enzymatic synthesis of penicillins J. Antibiot. 41: 1074-1084
M2080
Ferrero, M. A., A. Reglero, J. Martin-Villocorta, J. M. Fernandez-Canon, and J. M. Luengo 1990. V. Biosynthesis of benzylpenicillin (G), phenoxymethylpenicillin (V) and octanoylpenicillin (K) from glutathione S-derivatives J. Antibiot. 43: 684-691
M2081
Martin-Villacorta, J., A. Reglero, and J. M. Luengo 1991. Acyl-CoA: 6-APA acyltransferase from Penicillium chrysogenum studies on its hydrolytic activity J. Antibiot. 44: 108-110
M2096
Ramsay, B. A., D. Wang, C. Chavarie, and D. Rouleau 1991. Penicillin production in an inverse fluidized bed bioreactor J. Ferment. Bioeng. 72: 495-497
M268
Macdonald, K. D., J. M. Hutchinson, and W. A. Gillett. 1964. Properties of heterozygous diploids between strains of Penicillium chrysogenum selected for high penicillin yield. Antonie van Leeuwenhoek 30: 209-224.
M269
O'Sullivan, C. Y., and S. J. Pirt. 1973. Penicillin production by lysine auxotrophs of Penicillium chrysogenum. J. Gen. Microbiol. 76: 65-75.
M974
Ferrero, M. A., A. Reglero, H. Martinez-Blanco, M. Fernandez-Valverde, and J. M. Luengo 1991. In vitro enzymatic synthesis of new penicillins containing keto acids as side chains Antimicrob. Agents Chemother. 35: 1931-1932
 

Medium



36
POTATO DEXTROSE AGAR (PDA)
Diced potatoes200.0 g
Glucose20.0 g
Agar15.0 g
Distilled water1.0 L
Boil finely diced potatoes in 500 ml of water until thoroughly cooked, filter through cheesecloth and add water to filtrate to 1.0 L. The agar is dissolved in the filtrate by heating, and the glucose is added prior to sterilization.

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