Related information


BCRC Number: 17974
Organism: Sterolibacterium denitrificans
Author: Tarlera and Denner
History: << DSMZ << S. Tarlera, chol1
Other Collection No.: DSM 13999; ATCC BAA-354
Source: Denitrifying reactor treating sanitary landfill leakate
Growth Conditions: 30°C 14 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B3983
Frozen Vial for all NTD$ 6000 Order this item


Tarlera, S., and E. B. M. Denner. 2003. Sterolibacterium denitrificans gen. nov., sp. nov., a novel cholesterol-oxidizing, denitrifying member of the β-Proteobacteria. Int. J. Syst. Evol. Microbiol. 53 : 1085-1091.


solution A9 mL
solution B500 μL
solution C25 μl
solution D50 μl
solution E25 μl
Solution F500 μL
Prepare solution A, distribute approbiate amounts of cholesterol (e.g. 5 mg for 10 mL medium) and solution A in anaerobic tubes, and autoclave anaerobically under 80% N2 + 20% CO2. Solutions B and F are also autoclaved under these conditions. Solution C, D and E may be prepared aerobically. After cooling, add to 9 mL solution A: 500 μl sol. B, 500 μl sol. F, 25 μl (one drop) sol. C and E, each, and 50 μl sol. D under anaerobic conditions. Adjust pH to 7.0±0.2.
Solution A:
CaCl20.02 g
Distilled water200 mL
MgSO4·7 H2O0.10 g
NH4Cl0.10 g
NaNO30.16 g

Solution B:
Distilled water100 mL
KH2PO41.00 g

Solution C: Trace element solution SL-10
CoCl2·6H2O190.0 mg
Distilled water990.0 mL
CuCl2·2H2O2.0 mg
FeCl2·4H2O1.5 g
ZnCl270.0 mg
NiCl2·6H2O24.0 mg
Na2MoO4·2H2O36.0 mg
MnCl2·4H2O100.0 mg
HCl (25%; 7.7M)10.0 mL
H3BO36.0 mg
Trace element solution SL-10: First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 mL (This medium is modified from DSM #120: METHANOSARCINA MEDIUM)

Solution D: Vitamin solution
Biotin20 mg
Distilled water1000 mL
Nicotine amide25 mg
Thiamine-HCl · 2 H2O50 mg
Pyridoxamine-HCl10 mg
α-lipoic acid50 mg
Vitamin B1250 mg
Riboflavin50 mg
Pantothenic acid50 mg
p-aminobenzoic acid50 mg
Nicotinic acid25 mg
Folic acid20 mg
Stir for some hours, filter sterilize the solution.

Solution E: Selenite-tungstate solution
Distilled water1 L
Na2WO4·2H2O4 mg
Na2SeO3·5H2O3 mg
NaOH0.5 g

Solution F:
Distilled water100 mL
NaHCO38.40 g

Related information

Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 300193, Taiwan

Phone: +886-3-5223191


Copyright © 2018.BCRC All rights reserved.

web maintainance: +886-3-5223191 ext 593