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Bacteria



BCRC Number: 17974
Organism: Sterolibacterium denitrificans
Author: Tarlera and Denner
History: << DSMZ << S. Tarlera, chol1
Other Collection No.: DSM 13999; ATCC BAA-354
Source: Denitrifying reactor treating sanitary landfill leakate
Growth Conditions: 30°C 14 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B3983
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References



B3983
Tarlera, S., and E. B. M. Denner. 2003. Sterolibacterium denitrificans gen. nov., sp. nov., a novel cholesterol-oxidizing, denitrifying member of the β-Proteobacteria. Int. J. Syst. Evol. Microbiol. 53 : 1085-1091.
 

Medium



801
ANAEROBIC CHOLESTEROL MEDIUM
solution A9 mL
solution B500 μL
solution C25 μl
solution D50 μl
solution E25 μl
Solution F500 μL
Prepare solution A, distribute approbiate amounts of cholesterol (e.g. 5 mg for 10 mL medium) and solution A in anaerobic tubes, and autoclave anaerobically under 80% N2 + 20% CO2. Solutions B and F are also autoclaved under these conditions. Solution C, D and E may be prepared aerobically. After cooling, add to 9 mL solution A: 500 μl sol. B, 500 μl sol. F, 25 μl (one drop) sol. C and E, each, and 50 μl sol. D under anaerobic conditions. Adjust pH to 7.0±0.2.
Solution A:
CaCl20.02 g
Distilled water200 mL
MgSO4·7 H2O0.10 g
NH4Cl0.10 g
NaNO30.16 g

Solution B:
Distilled water100 mL
KH2PO41.00 g

Solution C: Trace element solution SL-10
CoCl2·6H2O190.0 mg
Distilled water990.0 mL
CuCl2·2H2O2.0 mg
FeCl2·4H2O1.5 g
ZnCl270.0 mg
NiCl2·6H2O24.0 mg
Na2MoO4·2H2O36.0 mg
MnCl2·4H2O100.0 mg
HCl (25%; 7.7M)10.0 mL
H3BO36.0 mg
Trace element solution SL-10: First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 mL (This medium is modified from DSM #120: METHANOSARCINA MEDIUM)

Solution D: Vitamin solution
Biotin20 mg
Distilled water1000 mL
Nicotine amide25 mg
Thiamine-HCl · 2 H2O50 mg
Pyridoxamine-HCl10 mg
α-lipoic acid50 mg
Vitamin B1250 mg
Riboflavin50 mg
Pantothenic acid50 mg
p-aminobenzoic acid50 mg
Nicotinic acid25 mg
Folic acid20 mg
Stir for some hours, filter sterilize the solution.

Solution E: Selenite-tungstate solution
Distilled water1 L
Na2WO4·2H2O4 mg
Na2SeO3·5H2O3 mg
NaOH0.5 g

Solution F:
Distilled water100 mL
NaHCO38.40 g

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