Bacteria



BCRC Number: 17705 Add To Bookmark
Organism: Campylobacter jejuni subsp. jejuni
Author: (Jones et al. ) Veron and Chatelain
History: << JCM << ATCC << N. R. Krieg VPI H840 << P. Dekeyser
Other Collection No.: JCM 2013; ATCC 29428; CCUG 39973; CCUG 41359; CIP 103778
Source: feces, human (diarrheic stool of child)
Characterization:
Assay : media testing --B3709;
Growth Conditions: 37°C 3 day(s)
Biosafety Level: 2
Oxygen Requirement: Microaerophilic
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References



B3709
. Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland): International Organization for Standardization ANSI; ISO ISO 11133-2:2003.

Medium



72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g
728
EG medium
Lab-lemco meat extract (Oxoid)2.4g
Proteose peptone No. 3 (Difco)10.0g
Yeast extract (Difco)5.0g
Na2HPO44.0g
Glucose1.5g
Soluble starch0.5g
L-Cysteine·HCl·H2O0.5g
L-Cystine0.2g
Agar15.0g
Horse blood50.0ml
Distilled water1.0L
Add L-cystine to 50 ml of 1N HCl and mix thoroughly. Add remaining components and bring volume to 950 ml. Adjust pH to 7.6-7.8. After autoclaving and then cooling to 50℃, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes.

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