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BCRC Number: 17660 Add To Bookmark
Organism: Alicyclobacillus acidoterrestris
Synonym: Bacillus acidoterrestris
Author: (Deinhard et al.) Wisotzkey et al.
History: << DSM << Deinhard, GD3B
Other Collection No.: SM 3922 ;ATCC 49025 ;DSM 3922
Source: Garden soil
Growth Conditions: 45°C 3 day(s)
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: YES
    Reference: B3705,B3706,B3707
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1988. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 25. Int. J. Syst. Bacteriol. 38: 220-222.
Deinhard, G., Blanz, P., Poralla, K., Altan, E. 1987. Bacillus acidoterrestris sp. nov., a new thermotolerant acidophile isolated from different soils. Syst. Appl. Microbiol. 10: 47-53.
Wisotzkey, J. D., Jurtshuk, P., Jr., Fox, G. E., Deinhard, G., Poralla, K. 1992. Comparative sequences analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int. J. Syst. Bacteriol. 42: 263-269.


Sterilize separately. For liquid medium combine solution A (with 1000.0 ml distilled water) andsolution B. For solid medium combine solution A (with 500 ml distilled water), solution B andsolution C. For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l.
Solution A
MgSO4 · 7 H2O0.50 g
(NH4)2 SO40.20 g
Yeast extract2.00 g
Glucose5.00 g
CaCl2 · 2 H2O0.25 g
KH2PO43.00 g
Distilled water (for liquid medium)1000.00 ml
Distilled water (for solid medium) 500.00 ml
Adjust medium for final pH 4.0 with H2SO4. Autoclave agar separately to avoid acid hydrolysis.

Solution B
Trace element sol. SL-61.00 ml

Solution C
Agar15.00 g
Distilled water500.00 ml

Solution B-Trace element sol. SL-6
ZnSO4 · 7 H2O0.10 g
MnCl2 · 4 H2O0.03 g
H3BO30.30 g
CoCl2 · 6 H2O0.20 g
CuCl2 · 2 H2O0.01 g
NiCl2 · 6 H2O0.02 g
Na2MoO4 · 2 H2O0.03 g
Distilled water1000.00 ml


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