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Bacteria



BCRC Number: 17574 Add To Bookmark
Organism: Tepidimonas ignava
Author: Moreira et al.
History: << DSMZ << M. Fernanda Nobre, SPS-1037
Other Collection No.: DSM 12034
Source: Hot spring Saó; Portugal.
Growth Conditions: 50°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: YES
    Reference: B3299
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References



B3299
Moreira, C., Rainey, F. A., Nobre, M. F., da Silva, M. T., and da Costa, M. S. 2000. Tepidimonas ignava gen. nov., sp. nov., a new chemolithoheterotrophic and slightly thermophilic member of the beta-Proteobacteria. Int. J. Syst. Evol. Microbiol. 50: 735-742.

Medium



629
THERMUS 162 MEDIUM
Yeast extract1.00g
Tryptone1.00g
Agar28.00g
Nitrilotriacetic acid100.00mg
CaSO4·2 H2O40.00mg
MgCl2·6 H2O200.00mg
0.01 M Fe citrate0.50ml
Trace element solution (see below)0.50ml
Phosphate buffer (see below)100.00ml
Distilled water900.00ml
Adjust pH to 7.2 with NaOH. Autoclave at 121℃ for 15 min. Autoclave the phosphate buffer separately and then add to the medium.
Phosphate buffer:
KH2PO45.44g
Na2HPO4·12 H2O43.00g
Distilled water1000.00ml
Adjust pH to 7.2

Trace element solution:
ZnSO4·7 H2O0.50g
H3BO30.50g
CuSO4·5 H2O25.00mg
Na2MoO4·2 H2O25.00mg
CoCl2·6 H2O45.00mg
H2SO40.50ml
MnSO4·H2O2.28g
Distilled water1000.00ml

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