Bacteria



BCRC Number: 17462 Add To Bookmark
Organism: Clostridium perfringens
Synonym: Clostridium welchii
Author: (Veillon and Zuber) Hauduroy et al.
History: << NCTC << B. Hobbs 3653\/50
Other Collection No.: NCTC 8239;ATCC 12917
Source: boiled salt beef
Others: ◆(2013-03-15)經測試後,於 BCRC medium 72 上生長良好。 ◆(2013-03-21)菌落有2種,一為灰色小顆光滑,一為透明霧狀,經16S rDNA序列分析結果菌名為/Clostridium perfringens/,兩種菌落相同,為正常現象。
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: Anaerobic
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References



B799
Craven SE. 1990. The effect of the pH of the sporulation environment on the heat resistance of Clostridium perfringens spores. Curr. Microbiol. 22: 233-237.

Medium



53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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