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BCRC Number: 17418 Add To Bookmark
Organism: Desulfovibrio cuneatus
Author: Sass et al.
History: << DSMZ << H.-D. Babenzien, STL1
Other Collection No.: DSM 11391
Growth Conditions: 28°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B784,B3069
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International Committee on Systematic Bacteriology. 1998. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 67. Int. J. Syst. Bacteriol. 48 : 1083–1084.
Sass, H., Berchtold, M., Branke, J., König, H., Cypionka, H., Babenzien, H.-D. 1998. Psychrotolerant sulfatereducing bacteria from an oxic freshwater sediment, description of Desulfovibrio cuneatus sp. nov. and Desulfovibrio litoralis sp. nov. Syst. Appl. Microbiol. 21: 212-219.


Dissolve the ingredients of each solution in the appropriate quantities of water. Bring solution A to the boil for a few minutes, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 in anaerobic tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C.
Solution A:
K2HPO40.5 g
CaCl2·2H2O0.1 g
MgSO4·7H2O2.0 g
Yeast extract1.0 g
Resazurin0.1 mg
NH4Cl1.0 g
Na2SO41.0 g
Distilled water980.0 ml
DL-Sodium lactate2.0 g

Solution B:
FeSO4·7H2O0.5 g
Distilled water10.0 ml

Solution C:
Ascorbic acid0.1 g
Distilled water10.0 ml
Sodium thioglycollate0.1 g


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