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Bacteria



BCRC Number: 17263 Add To Bookmark
Organism: Thermus filiformis
Author: Hudson et al.
History: << ATCC << J.A. Hudson and H.W. Morgan, Wai33 A1.
Other Collection No.: ATCC 43280; DSM 4687
Source: hot spring
Isolated Info:
    Isolated location: New Zealand
Growth Conditions: 70°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: YES
    Reference: B3542
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References



B3542
Hudson, J. A., Morgan, H. W., Daniel, R. M. 1987. Thermus filiformis sp. nov., a filamentous caldoactive bacterium. Int. J. Syst. Bacteriol. 37: 431-436.

Medium



501
CASTENHOLZ TYE MEDIUM
Castenholz Salts, 2·:
FeCl3 solution (0.03%)2.0ml
CaSO4·2H2O0.12g
MgSO4·7H2O0.2g
NaCl0.016g
KNO30.21g
Nitsch's Trace Elements (see below)2.0ml
Distilled weter1.0L
Nitrilotriacetic and0.2g
NaNO31.4g
Na2HPO40.22g
Agar (if needed)30.0g
Mix asptically 5 parts double strength Castenholz Salts (see below) with one part 1% TYE (see below) and 4 parts distilled water. Final pH of complete medium should be 7.6. Adjust pH to 8.2.

Nitsc's Trace Elements:
MnSO42.2g
Distilled water1.0L
CoCl2·6H2O0.046g
H2O0.5ml
ZnSO40.5g
H2BO30.5g
CuSO40.016g
Na2MoO40.025g

1% TYE
Tryptone (Difco 0123)10.0g
Yeast extract10.0g
Distilled water1.0L

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