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Actinomycetes



BCRC Number: 17230 Add To Bookmark
Organism: Mycobacterium neoaurum
Author: Tsukamura
History: << ATCC
Other Collection No.: ATCC 25795 ;NCTC 10818
Source: soil
Characterization:
Production : L-amino amidase -- B9174;
Growth Conditions: 37°C
Biosafety Level: 1
Shipping/Field/Price:
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References



B9174
Hermes HF, et al.. 1994. Purification and characterization of an L-amino amidase from Mycobacterium neoaurum ATCC 25795. Appl. Environ. Microbiol. 60: 153-159.

Medium



50
LOWENSTEIN MEDIUM, JENSEN (DIFCO 1017)
Glycerol12.0ml
Distilled water588.0ml
Homogenized egg1.0L
Lowenstein medium base (see below)37.2g
1.Suspend 37.2g of base in 600 ml distilled or deionized water containing 12 ml Bacto Glycerol and heat to boiling with constant agitation.
2.Sterilize in the autoclave for 15 minutes at 15 lbs pressure (121°C). Cool to 45-60°C.
3.Add the sterile base to 1 liter of a uniform suspension of fresh egg that was collected and suspended under aseptic conditions. Mix thoroughly,avoiding the inclusion of air bubbles.
4.Dispense the complete medium into sterile tubes or bottles,preferably screw capped.Arrange in a slant position in a suitable rack.
5.Coagulate the medium in an inspissator,water bath or autoclave at 85°C for 45 minutes.
6. You need for 1 l of egg mixture 20 - 25 fresh eggs (not older then 1 week). Clean the eggs, wipe away with 70% alcohol, open the eggs and homogenize the egg mixture carefully.
Lowenstein medium base:
L-asparagine3.6g
KH2PO42.4g
MgSO40.24g
Potato flour30.0g
Malachite green0.4g
Magnesium citrate0.6g

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