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Bacteria



BCRC Number: 17173 Add To Bookmark
Organism: Rubrobacter taiwanensis
Synonym:
Author: Chen et al.
History: << NTUB, M. Y. Chen, LS-293T
Other Collection No.: NTUB LS-293T ;JCM12932 ;ATCC BAA-406
Source: Hot springs
Isolated Info:
    Isolated location: Lu-Sang, Nangtou, Taiwan
Growth Conditions: 55°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
Local Strain: YES
Type Strain: YES
    Reference: B3396
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References



B3396
M. Y. Chen, S. H. Wu, G. H. Lin, C. P. Lu, Y. T. Lin, W. C. Chang, and S. S. Tsay. 2004. Rubrobacter taiwanensis sp. nov., a novel thermophilic, radiation-resistant species isolated from hot springs. Int. J. Syst. Evol. Microbiol. 54: 1849 - 1855.
Keyword
Rubrobacter taiwanensis

Medium



580
Modified Thermus medium
Yeast extract1g
Glutamatic acid (mono-sodium)1g
Agar20g
Peptone3g
Distilled water900ml
10X Castenholz salts100ml
Modified Thermus medium: adjust pH to 7.8 with NaOH Autoclaved at 121℃, 20 min
Castenholz Basal Salts (10X concentrate)
Nitrilo triacetic acid1.0g
MgSO4· 7H2O1.0g
NaCl0.08g
KNO31.03g
NaNO36.89g
FeCl3 soln (0.28 g/L)10.0ml
Nitsch's trace elements soln.10.0ml
CaSO4· 2H2O0.6g
Distilled water980.0ml
Na2HPO41.11g
Castenholz Basal Salts (10X concentrate): *Adjust pH value to 8.0 by 5N NaOH

Nitsch's trace elements soln. (100X concentrate)
H2SO40.5ml
MnSO4· H2O2.2g
H3BO30.5g
CoCl2· 6H2O0.046g
ZnSO4· 7H2O0.5g
CuSO40.016g
Na2MoO2· 2H2O0.025g
Distilled water1.0L
Nitsch's trace elements soln. (100X concentrate): 1L

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