Bacteria



BCRC Number: 17059
Organism: Pseudomonas putida
Author: (Trevisan) Migula
History: << ATCC << D. T. Gibson, F1
Other Collection No.: ATCC 700007
Source: soil
Characterization:
Degradation of : toluene --B3071, B3072, B3073, B3074;
benzene and ethylbenzene --B3071;
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
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References



B3071
Gibson, D. T., J. R. Koch, and R. E. Kallio. 1968. Oxidative degradation of aromatic hydrocarbons by microorganisms. I. Enzymatic formation of catechol from benzene. Biochemistry 7: 2653-2662.
B3072
Gibson, D. T., M. Hensley, H. Yoshioka, and T. J. Mabry. 1970. Formation of (+)-cis-2,3-dihydroxy-1-methylcyclohexa-4,6-diene from toluene by Pseudomonas putida. Biochemistry 9: 1626-1630.
B3073
Torok, D. S., S. M. Resnick, J. M. Brand, D. L. Cruden, and D. T. Gibson. 1995. Desaturation and oxygenation of 1,2-dihydronaphthalene by toluene and naphthalene dioxygenase. J. Bacteriol. 177: 5799-5805.
B3074
Zylstra, G. J. and D. T. Gibson. 1989. Toluene degradation by Pseudomonas putida F1. J. Biol. Chem. 264: 14940-14946.
 

Medium



3
NUTRIENT AGAR (DIFCO 0001)
Beef extract3.0 g
Peptone5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.0.

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