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Bacteria



BCRC Number: 17055 Add To Bookmark
Organism: Komagataeibacter intermedius
Synonym: Gluconacetobacter intermedius
Author: (Boesch et al.) Yamada et al.
History: << DSM << C. Boesch, ETH; TF2.
Other Collection No.: DSM 11804
Source: tea fungus beverage (Komucha)
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: YES
    Reference: B3068,B3069,B2123
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References



B2123
Yamada, Y., P. Yukphan, H. T. Lan Vu, Y. Muramatsu, D. Ochaikul, S. Tanasupawat, and Y. Nakagawa. 2012. Description of Komagataeibacter gen. nov., with proposals of new combinations (Acetobacteraceae). J. Gen. Appl. Microbiol. 58 : 397–404.
B3068
Boesch, C., J. Trcek, M. Sievers, and M. Teuber. 1998. Acetobacter intermedius, sp. nov. System. Appl. Microbiol. 21: 220-229.
B3069
International Committee on Systematic Bacteriology. 1998. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 67. Int. J. Syst. Bacteriol. 48 : 1083–1084.

Medium



1
MANNITOL AGAR
Yeast extract5.0g
Agar15.0g
Distilled water1.0L
Peptone3.0g
Mannitol25.0g
530
RAE MEDIUM
Glucose40.00g
Peptone10.00g
Yeast extract10.00g
Citric acid·H2O1.50g
Na2HPO4·2H2O3.38g
Glacial acetic acid10.00ml
Absolute ethanol10.00ml
Distilled water980.00ml
Final pH ca. 3.8 (pH is not adjusted) The ethanol and acetic acid are sterilized separately by filtration and added aseptically to the sterile medium. A double layer system has to be used. A layer of growth medium containing 1% agar is poured into a plate and allowed to solidify. A thin layer of the growth medium containing 2% agar is then poured on top.

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