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Bacteria



BCRC Number: 17046 Add To Bookmark
Organism: Legionella brunensis
Author: Wilkinson et al.
History: << ATCC << H. W. Wilkinson strain 441-1 << V. Drasar
Other Collection No.: ATCC 43878 ;CDC 1635-CZK-E
Source: cooling tower water, Czechoslovakia
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: 5% CO2
Type Strain: YES
    Reference: B3058,B3059
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References



B3058
1989. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 29. Int. J. Syst. Bacteriol. 39: 205-206.
B3059
Wilkinson, H. W., V. Drasar, W. L. Thacker, R. F. Benson, J. Schindler, B. Potuznikova, W. R. Mayberry, and D. J. Brenner. 1988. Legionella moravica sp. nov. and Legionella brunensis sp. nov. isolated from cooling-tower water. Ann. Inst. Pasteur Microbiol. 139: 393-402.

Medium



509
BCYE AGAR
L-Cysteine·HCl·H2O0.4g
Ferric phrophosphate soluble0.25g
Agar (if needed)17.0g
Distilled water1.0L
Yeast Extract (Difco 0127),10.0g
Charcoal (Sigma C5510)*2.0g
referencing to ATCC medium 1099: ATCC Culture medium 1099 CYE (buffered) (ATCC medium 1088) with THE ADDITION OF 10 GRAMS ACES*(N-2-ACETAMIDO-2-AMINOETHANE SULFONIC ACID) TO THE AUTOCLAVABLE FRACTION referencing to *Calbiochem # 104801 referencing to ATCC Medium 1088: *Activated, acid-washed with phosphoric and sulfuric acids. Adjust final pH to 6.90±0.05. Add all the ingredients of CYE agar except L-cysteine·HCl and soluble ferric pyrophosphate to 980ml distilled water; dissolve by boiling. Autoclave at 121℃ for 15 Prepare fresh solution of L-cysteine·HCl (0.40g in 10 ml distilled water) and soluble ferric pyrophosphate (0.25 g in 10 ml distilled water).Membrane filter-sterilize each solution separately. Add L-cysteine·HCl to basal medium first. Adjust the complet

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