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Bacteria



BCRC Number: 17043
Organism: Legionella anisa
Author: Gorman et al.
History: << ATCC << G.W. Gorman WA-316-C3
Other Collection No.: ATCC 35292 ;NCTC 11974
Source: tap water, Los Angeles, CA
Others: 1.(2016-06-29)The frozen vial possess fluorescent.
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: 5% CO2
Type Strain: YES
    Reference: B885,B3053
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References



B3053
Gorman, G. W., J. C. Feeley, A. Steigerwalt, P. H. Edelstein, C. W. Moss, and D. J. Brenner. 1985. Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower. Appl. Environ. Microbiol. 49: 305-309.
B885
1985. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 18. Int. J. Syst. Bacteriol. 35: 375-376.
 

Medium



509
BCYE AGAR
L-Cysteine·HCl·H2O0.4g
Ferric phrophosphate soluble0.25g
Agar (if needed)17.0g
Distilled water1.0L
Yeast Extract (Difco 0127),10.0g
Charcoal (Sigma C5510)*2.0g
referencing to ATCC medium 1099: ATCC Culture medium 1099 CYE (buffered) (ATCC medium 1088) with THE ADDITION OF 10 GRAMS ACES*(N-2-ACETAMIDO-2-AMINOETHANE SULFONIC ACID) TO THE AUTOCLAVABLE FRACTION referencing to *Calbiochem # 104801 referencing to ATCC Medium 1088: *Activated, acid-washed with phosphoric and sulfuric acids. Adjust final pH to 6.90±0.05. Add all the ingredients of CYE agar except L-cysteine·HCl and soluble ferric pyrophosphate to 980ml distilled water; dissolve by boiling. Autoclave at 121℃ for 15 Prepare fresh solution of L-cysteine·HCl (0.40g in 10 ml distilled water) and soluble ferric pyrophosphate (0.25 g in 10 ml distilled water).Membrane filter-sterilize each solution separately. Add L-cysteine·HCl to basal medium first. Adjust the complet

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