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Bacteria



BCRC Number: 17042 Add To Bookmark
Organism: Legionella birminghamensis
Author: Wilkinson et al.
History: << ATCC << H.W. Wilkinson 1407-AL-H << Univ. Alabama Sch. Med
Other Collection No.: ATCC 43702
Source: lung biopsy, Alabama
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: 5% CO2
Type Strain: YES
    Reference: B1099,B3052
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References



B1099
1988. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No.25. Int. J. Syst. Bacteriol. 38 : 220-222.
B3052
Wilkinson, H. W., W. L. Thacker, R. F. Benson, S. S. Polt, E. Brookings, W. R. Mayberry, D. J. Brenner, R. G. Gilley, and J. K. Kirklin. 1987. Legionella birminghamensis sp. nov. isolated from a cardiac transplant recipient. J. Clin. Microbiol. 25: 2120-2122.

Medium



509
BCYE AGAR
L-Cysteine·HCl·H2O0.4g
Ferric phrophosphate soluble0.25g
Agar (if needed)17.0g
Distilled water1.0L
Yeast Extract (Difco 0127),10.0g
Charcoal (Sigma C5510)*2.0g
referencing to ATCC medium 1099: ATCC Culture medium 1099 CYE (buffered) (ATCC medium 1088) with THE ADDITION OF 10 GRAMS ACES*(N-2-ACETAMIDO-2-AMINOETHANE SULFONIC ACID) TO THE AUTOCLAVABLE FRACTION referencing to *Calbiochem # 104801 referencing to ATCC Medium 1088: *Activated, acid-washed with phosphoric and sulfuric acids. Adjust final pH to 6.90±0.05. Add all the ingredients of CYE agar except L-cysteine·HCl and soluble ferric pyrophosphate to 980ml distilled water; dissolve by boiling. Autoclave at 121℃ for 15 Prepare fresh solution of L-cysteine·HCl (0.40g in 10 ml distilled water) and soluble ferric pyrophosphate (0.25 g in 10 ml distilled water).Membrane filter-sterilize each solution separately. Add L-cysteine·HCl to basal medium first. Adjust the complet

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