Bacteria



BCRC Number: 16407 Add To Bookmark
Organism: Rhodobacter sphaeroides
Author: (van Niel) Imhoff et al.
History: << ATCC << C.B. van Niel ATH 2.4.1
Other Collection No.: ATCC 17023 ;ATCC 11167 ;ATCC 14690 ;NCIB 8253 ;NCIB 8287 ;DSM 158
Others: 1.(2018-01-18)好氧及厭氧條件下皆可活化培養。
2.(2018-01-18)好氧條件:好氧-黑暗-28℃-培養基 No. 3, 66, 982.
3.(2018-01-18)厭氧條件:厭氧(以厭氧罐培養)-照光(tungsten lamp)-25℃-培養基 No. 903
4.(2018-01-18)冷凍甘油管置於37℃溶解後,進行活化培養,不可重複使用,易失去活性。
Category: photosynthetic bacterium
Growth Conditions: 28°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: YES
    Reference: B2207,B2208
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References



B2207
Pfennig, N. and H. G. Truper. 1971. Type and neotype strains of the species of phototrophic bacteria maintained in pure culture. Int. J. Syst. Bacteriol. 21 : 19–24.
B2208
Pike, L. and G. A. Sojka. 1975. Glycerol dissimilation in Rhodopseudomonas sphaeroides. J. Bacteriol. 124: 1101-1105.

Medium



3
NUTRIENT AGAR (DIFCO 0001)
Beef extract3.0 g
Peptone5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.0.
66
TRYPTIC SOY BROTH OR TRYPTIC SOY AGAR (DIFCO 0369)
Tryptone15.0 g
Soytone5.0 g
NaCl5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.3.
903
RHODOSPIRILLACEAE MEDIUM (modified)
Yeast extract0.30 g
L-Cysteiniumchloride0.30 g
Na2-succinate1.00 g
(NH4)-acetate0.50 g
Fe(III) citrate solution (0.1% in H2O)5.00 mL
KH2PO40.50 g
MgSO4 · 7 H2O0.40 g
NaCl0.40 g
NH4Cl0.40 g
CaCl2 · 2 H2O0.05 g
Vitamin B12 solution (10 mg in 100 mL H2O)0.40 mL
Trace element solution SL-6 (see below)1.00 mL
Resazurin (0.1%)0.50 mL
Distilled water1000 mL
Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.
Trace element solution SL-6
ZnSO4·7 H2O0.10 g
MnCl2·4 H2O0.03 g
H3BO30.30 g
CoCl2· 6 H2O0.20 g
CuCl2 ·2 H2O0.01 g
NiCl2· 6 H2O0.02 g
Na2MoO4·2 H2O0.03 g
Distilled water1000 mL

none

982
Hipolypepton medium
Hipolypepton10 g
Yeast extract2 g
MgSO4·7H2O1 g
Distilled Water1 L
Agar (if needed)15 g
Adjust pH to 7.0. Hipolypepton is from Wako Pure Chemical Ind., Ltd., Osaka, Japan.

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