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Archaea



BCRC Number: 16173 Add To Bookmark
Organism: Methanothrix soehngenii
Synonym: Methanothrix concilii, Methanosaeta concilii
History: << DSM << B. Schink << B. A. Huser
Author: Patel and Sprott
Other Collection No.: DSM 2139
Source: anaerobic sewage sludge, Switzerland
Others: Only supply active culture (Liquid culture, 2 bottles, each 50 mL)。
Growth Conditions: 37°C 21 day(s)
Oxygen Requirement: Strictly Anaerobic
Biosafety Level: 1
Type Strain: YES
    Reference: B000363
Shipping/Field/Price:
Liquid Culture for all NTD$ 30000 Order this item


References



B000363
Huser, B.A., Wuhrmann, K. & Zehnder, A.J.B. 999. Methanothrix soehngenii gen. nov. sp. nov., a new acetotrophic non-hydrogen-oxidizing methane bacterium. Arch. Microbiol. 132: 1–9.

Medium



551
METHANOTHRIX MEDIUM
NaCl0.6g
MgCl2 . 6H2O0.1g
CaCl2 . 2H2O0.08g
Trace element solution 10.0ml
Vitamin solution 10.0ml
NH4Cl1.0g
KH2PO40.3g
Resazurin1.0mg
KHCO34.0g
Distilled water1.0L
sodium acetate 6.8g
Cysteine-HCl . H2O0.3g
Na2S . 9H2O0.3g
Final pH should be 7.0. Use 20% inoculum! Pressurize culture bottles to 1 bar overpressure with 80% N2 + 20% CO2.
Trace element solution
Nitrilotriacetic acid12.8g
FeCl3 . 6H2O1.35g
MnCl2 . 4H2O0.1g
CoCl2 . 6H2O0.024g
CaCl2 . 2H2O0.1g
ZnCl20.1g
CuCl2 . 2H2O0.025g
H3BO30.01g
Na2MoO4 . 2H2O0.024g
NaCl1.0g
NiCl2 . 6H2O0.12g
Na2SeO3 . 5H2O0.026g
First dissolve Nitrilotriacetic acid in 200 ml of water and adjust the pH to 6.5 with KOH.

Vitamin solution
Biotin2.0mg
Folic acid2.0mg
Pyridoxine-HCl10.0ml
Thiamine-HCl . 2H2O5.0mg
Riboflavin5.0mg
Nicotinic acid 5.0mg
D-Ca-pantothenate5.0mg
Vitamin B12 0.1mg
p-Aminobenzoic acid5.0mg
Lipoic acid5.0mg
Distilled water1.0L
Sterilize the vitamins by filtration.

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