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Archaea



BCRC Number: 16169 Add To Bookmark
Organism: Methanofollis tationis
Synonym: Methanogenium tationis
History: << DSM << J. Winter
Author: Zellner et al.
Other Collection No.: DSM 2702; OCM 159
Source: mud from hot solfataric pool
Others: Only supply active culture (Liquid culture, 2 bottles, each 50 mL)。
Growth Conditions: 37°C
Oxygen Requirement: Strictly Anaerobic
Biosafety Level: 1
Type Strain: YES
    Reference: B2316
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References



B2316
Zellner, G., D. R. Boone, J. Keswani, W. B. Whitman,C. R. Woese, A. Hagelstein, B. J. Tindall, and E. Stackebrandt. 1999. Reclassification of Methanogenium tationis and Methanogenium liminatans as Methanofollis tationis gen. nov., comb. nov. and Methanofollis liminatans comb. nov. and description of a new strain of Methanofollis liminatans. Int. J. Syst. Bacteriol. 49: 247-255.

Medium



547
METHANOBACTERIUM MEDIUM
NaCl0.40g
NH4Cl0.40g
CaCl2·2H2O0.05g
Yeast extract1.0g
sodium acetate5.0g
sodium formate2.0g
Sludge fluid 50.0ml
NaHCO34.0g
Fatty acid mixture 20.0ml
NiCl2·6H2O 24.0mg
KH2PO40.50g
MgSO4·7H2O0.40g
FeSO4·7H2O0.002g
Trace element solution SL-101.00ml
Resazurin0..001 g
Distilled water 940.0ml
Adjust pH to 6.7 - 7.0. Prepare the medium anaerobically under a gas atmosphere of 80% H2 and 20% CO2.
Trace element solution SL-10
HCl (25%; 7.7 M)10.0ml
FeCl2·4H2O1.50g
ZnCl270.0mg
MnCl2·4H2O100mg
H3BO36.0mg
CoCl2·6H2O190.0mg
CuCl2·2H2O2.0mg
Na2MoO436.0mg
Distilled water990ml
NiCl2·6H2O24.0mg
First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Sludge fluid
Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37°C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark.

Fatty acid mixture
Valeric acid 0.50g
Isovaleric acid 0.50g
2-Methylbutyric acid 0.5g
Isobutyric acid 0.50g
Distilled water 20ml
Adjust pH to 7.5 with conc. NaOH.

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