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BCRC Number: 16166 Add To Bookmark
Organism: Methanofollis aquaemaris
History: << NCHUB N2F9704 << M.C. Lai
Author: Lai and Chen
Other Collection No.: DSM 14661; OCM 746
Source: aquaculture fishpond, Taiwan
Growth Conditions: 37°C
Oxygen Requirement: Anaerobic
Biosafety Level: 1
Local Strain: YES
Type Strain: YES
    Reference: B3268
Liquid Culture for all NTD$ 30000 Order this item


Boone, D. R. and W. B. Whitman. 1988. Proposal of minimal standards for describing new taxa of methanogenic bacteria. Int. J. Syst. Bacteriol. 38: 212-219.
Garcia, J. L. 1990. Taxonomay and ecology of methanogens. FEMS Microbiol. Rev. 87: 297-308.
Lai, M.-C. and S.-C. Chen. 2001. Methanofollis aquaemaris sp. nov., a methanogen isolated from an aquaculture fish pond. Int. J. Syst. Evol. Microbiol. 51: 1873-1880.


MB MEDIUM with 100 mM Formate
CaCl2·2H2O0.4 g
Cysteine ·HCl0.25 g
Distilled water980.0 mL
K2HPO40.4 g
KCl0.5 g
MgCl2·6H2O1.0 g
Na2S·9H2O0.24 g
NaHCO34.0 g
NH4Cl1.0 g
Resazurin0.1 mg
Sodium formate6.8 g
Trace element(see below)10.0 mL
Tryptone2.0 g
Vitamin solution (see below)10.0 mL
Yeast extract2.0 g
The medium refer to medium 385 is prepared anaerobically under an atmosphere of 80% N2+20% CO2. Formate and Na2S·9H2O (reducing agents) are sterilized separately under N2 atmosphere as concentrated.
Vitamin solution (should be stored at -20°C)
Biotin2.0 mg
Distilled water1.0 L
Nicotinic acid5.0 mg
Folic acid2.0 mg
p-Aminobenzonic acid5.0 mg
Pyridoxin hydrochloride10.0 mg
Thiamine-HCl x 2 H2O5.0 mg
Vitamin B120.1 mg
Thioctic acid5.0 mg
Riboflavin5.0 mg
Pantothenic acid5.0 mg

Trace element solution
AlCl3·6H2O0.04 g
CoCl2·6H2O0.15 g
H2SeO30.01 g
MnCl2·4H2O0.10 g
ZnCl20.10 g
NiSO4·6H2O0.02 g
NaMoO4·2H2O0.01 g
H3BO30.01 g
FeSO4·7H2O0.10 g
Distilled water1.0 L
CuCl2·2H2O0.02 g
Disodium EDTA dihydrate0.50 g


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