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BCRC Number: 16146 Add To Bookmark
Organism: Cutibacterium acnes
Synonym: Propionibacterium acnes
Author: Scholz and Kilian, comb. nov.
History: << ATCC << H. Seeliger 417-52(Corynebacterium liquefaciens)
Other Collection No.: ATCC 11828
Source: subcutaneous abscess
Others: 1.cell wall and DNA analyses--B184
2.經測試後,於 BCRC medium 72, 228 上生長良好,亦可使用此二種培養基。
3.Rename as ATCC 6919 and Ref no. B000122
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: Anaerobic
Freeze-Dried for all NTD$ 5000 Order this item



GAM broth (Nissui)59.0 g
Agar2.0 g
Distilled water1.0 L
0.025% Resazurin solution4.0 mL
1N NaOH25.0 mL
Agar (if necessary)20.0 g
Cysteine·HCl0.5 g
Distilled water1.0 L
Ground beef (fat free)500.0 g
Hemin solution (see below)10.0 mL
K2HPO45.0 g
Trypticase peptone30.0 g
Vitamin K1 solution (see below)0.2 mL
Yeast extract5.0 g
Mix meat, water and NaOH and bring to a boil with stirring. Cool to room temperature, skim fat from surface, and filter, retaining both meat particles and filtrate. To filtrate, add sufficient distilled water to restore volumn to 1.0 L. To this filtrate, add trypticase peptone, yeast extract, K2HPO4, resazurin solution and agar (if necessary). Boil and cool medium under 80% N2, 10% CO2, and 10% H2, then add Cysteine-HCl, Hemin solution and Vitamin K1 solution. Adjust pH to 7.0. Anaerobically dispense 7 mL into tubes containing meat particles (1 part meat to 5 parts fluid) under the same gas phase.
Hemin solution:
Dissolve 50 mg hemin in 1mL 1N NaOH, make up to 100 mL with distilled water. Store refrigerated.

Vitamin K1 solution:
Dissolve 0.15 mL of Vitamin K1 in 30 mL of 95% ethanol and filter sterile. Keep Vitamin K1 solution under refrigeration. Store in brown bottle.

TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g


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