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Bacteria



BCRC Number: 16136 Add To Bookmark
Organism: Collinsella aerofaciens
Synonym: Eubacterium aerofaciens
Author: (Eggerth) Kageyama et al
History: << VPI 1003
Other Collection No.: ATCC 25986; DSM 3979
Source: human feces, U.S.A.
Category: anaerobe
Growth Conditions: 37°C 3 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B3261
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
B3261
Kageyama, A., Y. Benno, and T. Nakase. 1999. Phylogenetic and phenotypic evidence for the transfer of Eubacterium aerofaciens to the genus Collinsella as Collinsella aerofaciens gen. nov., comb. nov. Int. J. Syst. Bacteriol. 49 : 557-565.
Keyword
Collinsella,Eubacterium aerofaciens, Collinsella aerofaciens

Medium



191
CHOPPED MEAT MEDIUM with Carbohydrates I
0.025% Resazurin solution4.0 mL
1N NaOH25.0 mL
Cellobiose1.0 g
Distilled water1.0 L
Glucose4.0 g
Ground beef (fat free)500.0 g
K2HPO45.0 g
L-cysteine·HCl0.5 g
Maltose1.0 g
Peptone30.0 g
Starch1.0 g
Yeast extract5.0 g
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, filter and retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1.0 L original volume. To the filtrate add peptone, yeast extract, K2HPO4 and resazurin solution. Boil, then cool under O2-free nitrogen and 3% hydrogen, and add 0.5 g L-cysteine·HCl. Adjust pH to 7.0. Dispense 4-5 parts fluid to 1 part meat particles per test tube. Cap with rubber stoppers under N2 and H2 and autoclave in press for 15 minutes under fast exhaust. Add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, and 0.1% soluble starch before adding cysteine.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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