Bacteria



BCRC Number: 15784
Organism: Methylophilus methylotrophus
Author: Jenkins et al.
History: << ATCC << Celanese Coporation
Other Collection No.: ATCC 53528
Characterization:
Ultilization methanol
Others: This material is cited in a U.S. and or other Patent Application and may not be used to infringe the patent claims.
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: YES
    Reference: B2049
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References



B2049
Jenkins, O., D. Byrom and D. Jones. 1987. Methylophilus: a new genus of methanol-utilizing bacteria. Int. J. Syst. Bacteriol. 37: 446-448.
 

Medium



512
METHANOL AMMONIUM SALTS MEDIUM
MgSO4·7H2O1.0 g
KH2PO40.26 g
Fe-EDTA5.0 mg
Na2MoO4·2H2O2.0 mg
NH4Cl0.5 g
MnCl2·4H2O20.0 μg
H3BO415.0 μg
CoCl2·6H2O50.0 μg
NiCl2·6H2O10.0 μg
EDTA250.0 μg
Distilled water1.0 L
CaCl20.2 g
Na2HPO40.33 g
FeSO4·7H2O500.0 μg
ZnSO4·7H2O400.0 μg
Adjust pH to 6.8. Sterilize the phosphates separately and add to the bulk of the medium when cool. Add sterile methanol to a concentration of 0.5%.

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