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Bacteria



BCRC Number: 14685
Organism: Alicyclobacillus acidocaldarius
Synonym: Bacillus acidocaldarius
Author: (Darland and Brock) Wisotzkey et al.
History: << DSM << ATCC << T. D. Brock 104-1A
Other Collection No.: ATCC 27009 ;DSM 446 ;NCIB 11725 ;IFO 15652 ;JCM 5260
Source: acid hot spring, U. S. A.
Others: ◆thermoacidophilic amylase
◆(2013-03-29)原始培養方法使用 BCRC medium 391 及 55℃ 培養。經測試後,於 BCRC medium 917 及 60℃ 環境下生長良好,建議用後者條件培養。
Growth Conditions: 60°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: YES
    Reference: B1,B3159
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
B3159
Wisotzkey, J. D., Jurtshuk, P. Jr., Fox, G. E., Deinhard, G., Poralla, K. 1992. Comparative sequences analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int. J. Syst. Bacteriol.
 

Medium



391
Bacillus medium
KH2PO40.37 g
MgSO4·7H2O0.25 g
CaCl2·2H2O0.07 g
FeCl30.02 g
Glucose1.0 g
(NH4)2SO41.3 g
Yeast extract1.0 g
distilled water1.0 L
Adjust pH to 4.0 with 10N H2SO4. For solid medium, make up above solution in double strength and adjust pH to 3.5. Make water agar at 40 g/L. Autoclave separately. Cool to about 50℃ and mix equal volumes of both solutions aseptical
917
ALICYCLOBACILLUS MEDIUM
Solutions A and B are separately sterilized by autoclaving and mixed.
Solution A:
MgSO4 x 7H2O 0.5 g
CaCl2 x 2H2O 0.25 g
KH2PO40.6 g
Distilled water 500 ml
Yeast extract1.0 g
(NH4)2SO40.2 g
Adjust pH to 3.0-4.0

Solution B:
Glucose1.0 g
Agar (if needed)20.0 g
Distilled water 500 ml

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